Search results for "Hybridation in situ"
showing 2 items of 2 documents
Biodiversity-functioning relations in microorganisms
2006
The role of microorganisms as key components in the functioning of ecosystems and biodiversity is discussed. The latest methodologies developed to study microbial diversity and functioning of ecosystems are considered, as DNA extraction and isotope labeling, fluorescent in situ hybridization and microautoradiography, and tracking of a substrate labeled with a stable isotope. Investigations related to correlations between microbial diversity and functioning in situ are considered. An example is illustrated of a correlation of erosion of microbial diversity of natural microbial community in prairie soil and erosion of species diversity in an ecosystem.
Etude et caractérisation de l'état " Viable mais Non Cultivable " chez Brettanomyces, une levure d'altération des vins : nouvel outil de détection et…
2011
The viable but not culturable (VBNC) state has been studied in detail in bacteria. It has been suggested that the VBNC state also exists in eukaryote cells, such as wine yeasts, including Brettanomyces in particular. We investigated the VBNC state in this yeast, focusing on the conditions for entry and exit, and the morphological and metabolic modifications associated with this state. We added sulfite (0.8 mg.L-1 molecular SO2) to induce the VBNC state. Increasing the pH of the medium inactivated the sulfite, allowing the cells to exit from the VBNC state and to become culturable again. In these conditions, we found that Brettanomyces VBNC cells were smaller than culturable cells, and that …